Offres de stage et propositions de thèse
Single-molecule fluorescence lifetime imaging nanoscopy for biophysics and thermoplasmonics

Domaines
Biophysics
Soft matter
Nanophysics, nanophotonics, 2D materials and van der Waals heterostructures,, surface physicss, new electronic states of matter

Type de stage
Expérimental
Description
Super-resolution imaging techniques enable to image objects with a resolution at the nanometer length scale (~10 nm), well below the classical limit imposed by the diffraction of light (~200 nm). Among these techniques, single-molecule localization microscopy (SMLM) approaches are based on the capacity of detecting single-molecules and the ability of switching on and off fluorescent emitters. At Institut Langevin, ESPCI Paris PSL, we have further developed such concept and conceived a novel microscopy system capable of simultaneously detecting single fluorescent molecules as well as their fluorescence lifetime, and thus obtaining super-resolved fluorescence lifetime images (smFLIM). So far, we have studied light-matter interactions in plasmonic and dielectric nanostructures and obtained super-resolved images of the local density of electromagnetic states of silver nanowires, gold nanocones and GaP nanodimers. We are looking for a motivated student to work with us to explore a new application of smFLIM, that is the study of temperature increase in nanostructured samples based on single-molecule fluorescence modification. smFLIM has the potential to address this challenge, by using stochastically photoactivatable molecules that have a temperature-dependent fluorescence. The sensitivity of single-molecules’ fluorescence to this parameter will be explored in the internship and its application to the map of plasmon induced temperature enhancement will be investigated.

Contact
Valentina Krachmalnicoff
+33180963073


Email
Laboratoire : Institut Langevin - UMR 7587
Equipe : Subwavelength Physics (SWAP)
Site Web de l'équipe
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