This internship, hosted at IJCLab (CNRS / Université Paris-Saclay) in collaboration with ISMO, aims to study how individual cancer cells respond to X-ray irradiation in the presence of metallic nanoparticles (NPs) that enhance radiotherapy effects. Despite advances in radiotherapy, tumor heterogeneity limits treatment efficacy. Metallic NPs can amplify tumor toxicity without increasing the delivered dose, but their precise biological effects remain poorly understood. Using quantitative videomicroscopy and the CellLineageTrack (CLT) algorithm developed at IJCLab, the project will track single cells over time to reconstruct lineage trees and characterize key parameters such as cell-cycle duration, motility, senescence, and survival. These dynamic observations will be integrated with ISMO’s expertise in nanoparticle radiosensitization to map multi-scale cellular responses to irradiation in real time. The internship combines experimental work (cell culture, nanoparticle exposure, real-time imaging under X-ray irradiation) and data analysis (image processing, Python coding, algorithm improvement). It is intended for a motivated Master’s student with a background in physics, strong interest in biophysics and cell biology, and solid Python programming skills.